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High Performance Liquid Chromatography Techniques

High performance liquid chromatography is central to pharmaceutical, food, water and petrochemical testing throughout African laboratories, yet method transfer between instruments and operators often introduces variability. This course develops sound HPLC practice, covering mobile phase preparation, column chemistry, detector selection and system suitability testing, so analysts generate consistent, defensible results across shifts and sites.

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What you will be able to do

  • Explain the principles of liquid chromatography separation and common detector technologies.
  • Prepare mobile phases and select columns appropriate to the analyte and matrix.
  • Perform system suitability testing before releasing quantitative HPLC results.
  • Operate HPLC systems for isocratic and gradient methods across sample types.
  • Troubleshoot pressure faults, peak shape problems and retention time drift.
  • Validate transferred methods to confirm equivalent performance between instruments and operators.

Course outline

  1. 01Liquid Chromatography Separation Principles
  2. 02Mobile Phase Preparation and Degassing
  3. 03Column Chemistry and Selection
  4. 04Detector Types and Selection Criteria
  5. 05Gradient and Isocratic Method Operation
  6. 06System Suitability Testing
  7. 07Troubleshooting Pressure and Peak Faults
  8. 08Method Transfer Between Instruments

Who should attend

This course is intended for HPLC analysts, method development chemists, quality control staff and instrument technicians working in pharmaceutical, food, water or petrochemical laboratories.

Dates, venues and fees

DatesVenueDaysFeeCalendarRegister
28 to 30 Sep 2026Lusaka3US$1,500Closed
29 Sep to 1 Oct 2026Harare3US$1,500Register
30 Sep to 2 Oct 2026Lilongwe3US$1,500Register
5 to 7 Oct 2026Blantyre3US$1,500Register
6 to 8 Oct 2026Mozambique3US$1,500Register
7 to 9 Oct 2026Nairobi3US$1,500Register
12 to 14 Oct 2026Dar es Salaam3US$1,500Register
13 to 15 Oct 2026Kigali3US$1,500Register
14 to 16 Oct 2026Kampala3US$1,500Register
19 to 21 Oct 2026Accra3US$1,500Register
20 to 22 Oct 2026Lagos3US$1,500Register
21 to 23 Oct 2026Cairo3US$1,500Register
26 to 28 Oct 2026Mauritius3US$1,500Register
27 to 29 Oct 2026Dubai3US$1,500Register
28 to 30 Oct 2026Online3US$900Register
24 to 26 Nov 2026Johannesburg3R13,000Register
25 to 27 Nov 2026Cape Town3R13,000Register
30 Nov to 2 Dec 2026Durban3R13,000Register
1 to 3 Dec 2026Pretoria3R13,000Register
2 to 4 Dec 2026Windhoek3US$1,500Register
7 to 9 Dec 2026Gaborone3US$1,500Register
8 to 10 Dec 2026Maseru3US$1,500Register
9 to 11 Dec 2026Mbabane3US$1,500Register

Fees are per delegate. South African venues are priced in rand; other locations and online in US dollars. Fees exclude 15% VAT where applicable. Group bookings of three or more delegates from the same organisation qualify for a reduced rate; contact us for a quotation.

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